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Research Technician

Position Information

Position Information

Date Posted 03/13/2018
Open Until Filled No
Anticipated Application Deadline (Close date) 05/13/2018
Working Title Research Technician
Job Classification Research III
Department Name 35215-Med-Orthopedic Surgery
Job Type Staff
Position Number 0134913
Hiring Range TBD based on qualifications
Position Description

The Center for Cellular and Molecular Engineering (CCME) of the University of Pittsburgh is seeking a Research Technician.

As a Research Technician in this lab, the incumbent will be a part of an exciting state-of-art internationally recognized tissue and cellular engineering research program in the area of musculoskeletal research, with a specific focus on cartilage and bone. The position is supported by a funded project and the Research Technician will work under the direct supervision of the senior scientists on the project. The incumbent will manage cell and tissue cultures and assess cell proliferation, differentiation, and metabolism using RT_PCR, biochemistry, histology, immunohistochemistry, and immunofluorescence (from sample preparation, to sectioning, to staining, etc). A major focus of the research will be on the development of 3D microphysiological constructs maintained in unique bioreactor systems and scaffolds developed in the lab.

The Research Technician is expected to maintain accurate records of laboratory procedures and results. Operating and maintaining sensitive laboratory equipment is also part of the job. The prompt communication of results, issues, and needs with team members is necessary. The incumbent must have a good knowledge of laboratory skills and a high level of self-sufficiency. The capacity to work independently (including the design and execution of experiments) with some direction from senior scientists is required. Experience in literature searches, identification of existing protocols and establishment/adaptation of protocol for specific cell culture conditions are expected as well as modifying standard procedures and methods to address the specific conditions of this project. Experience with mammalian cells culture is required. Experience in using optical microscopes in bright field mode and in fluorescence mode is required. Experience in either RT PCR or tissue histology/immunohistochemistry is required.

This project requires the use of biochemical, histo/immune-histochemical and molecular techniques including, but not limited to, MTT, DMMB, alkaline phosphatase, TRAP, and hydroxyproline assays, DNA/RNA preparation and handling, RT PCR, western blotting and ELISAs, microscopy, basic histological staining, and immunohistochemistry (including immunofluorescence). Hence, the capacity to perform laboratory procedures and tests is expected. The ability to use the Office suite, including Excel, is required, as is the capacity to perform the basic calculations for dilutions, standard curves, and quantification of data obtained by assays such as RT PCR or colorimetric assays. Multi-tasking several experiments simultaneously and efficient time management is necessary. Many of the responsibilities described above will be self-initiated and require a high level of vigilance. Meticulous work habits, dependability, and good communication skills are required. Two years experience of wet lab work are required.

Cover letter should describe research experience, long-term career goals, and why you would be a good fit for this position.

This is position is subject to funding.

Education Required Baccalaureate
Minimum Related Experience Level Required 2-3 years experience
Additional Education and/or Experience Essential to the Position

Bachelor’s Degree or equivalent in biology, chemistry, molecular biology, biochemistry, bioengineering, or related field is required. Experience in mammalian cell culture is required. Experience in optical microscopes (bright field/fluorescence) is required. Experience in either RT PCR or tissue histology/immunohistochemistry is required, emphasis in musculoskeletal tissues/cells is an asset. Three years of experience is preferred.

Required Licenses/Certifications

All regulatory compliance modules regarding laboratory safety, EHS, etc. before beginning in the lab (will be provided locally).

Child Protection Clearances

The following PA Act 153 clearances and background checks may be required prior to commencement of employment and as a condition of continued employment: PA State Police Criminal Record Check, FBI Criminal Record Check, PA Child Abuse History Clearance.

Background Check For position finalists, employment with the University will require successful completion of a background check.
Assignment Category Regular, Full-Time
Percent Effort 100
Scheduled Work Hours Work Schedule to Vary
If Varied Work Schedule, Please Specify

Work schedule may vary – typical work schedule will be Monday – Friday 9:00-5:30; however, other hours/days may be necessary based on experiment schedule and deadlines.

If Part Time, Actual Standard Weekly Hours
Campus Pittsburgh
University Benefits

The University offers an excellent comprehensive benefits package for all full-time and part-time regular employees including:

• Education benefits and a retirement plan with employer match

• Group medical insurance, life insurance, and optional vision and dental insurance

• Free transit service within Allegheny County for employees of the Oakland campus (Port Authority Transit)

• Time off benefits including vacation, sick and personal time

For more information regarding the University’s benefits package, please visit

Supplemental Questions

Required fields are indicated with an asterisk (*).

  1. * Position has funding through September 2018, thereafter, the funding is not certain. Are you willing to take this position knowing there is guaranteed funding only through September 2018.
    • Yes
    • No
  2. * How did you hear of this position at the University of Pittsburgh?
    • Career Development Center (CDC)
    • Chronicle of Higher Education
    • Commonwealth Workforce Development System (Formerly PA CareerLink)
    • Currently a University Employee
    • Pitt Career Services
    • Pittsburgh Post Gazette
    • Pittsburgh Technology Council
    • PittSource Search
    • Referral (Friend, University Employee, etc.), please specify below
    • Internet Search
    • Other: (Please specify below)
  3. Please specify from above (if applicable):

    (Open Ended Question)

Applicant Documents

Required Documents
  1. Resume
Optional Documents
  1. Cover Letter
  2. Curriculum Vitae